根据前期耐盐芯片研究提供的两条EST序列设计引物,利用RACE技术从番茄耐盐品种Edkawi中克隆了其5′和3′片段,并拼接成全长cDNA,分别命名为SlSRG1和SlSRG2.两个基因序列在GenBank中的登录号为EU670751和EU670752,其大小分别为1300 bp和1810 bp,编码蛋白分别为309和499个氨基酸.半定量RT-PCR表明SlSRG1在番茄茎、叶、花中表达较强,在所检测的其它组织中表达很弱,SlSRG2在叶和花中表达量最高,其次为茎和根,在果实中表达微弱.盐胁迫表达谱结果显示SlSRG1在盐处理的Edkawi中缓慢增强,SlSRG2则在盐胁迫后表达迅速增强,在未进行盐胁迫的对照中,两个基因的表达趋势均为减弱.本研究为番茄抗逆研究提供了新的候选基因资源.%Based on the ESTs derived from previous microarray results under salt stress, the full-length cDNAs of two salt-inducible genes ( SISRG1, SISRG2) were isolated from a salt tolerant tomato cultivar, Edkawi, using Rapid Amplification of cDNA Ends (RACE). The accession numbers of SISRG1 and SISRG2 in GenBank are EU670751and EU670752, respectively. The corresponding cDNAs are 1300 bp and 1810 bp in length and the deduced proteins contain 287 and 499 amino acids, respectively. Tissue expression profile analysis using semiquantitative RT-PCR showed that SISRG1 was expressed mainly in the stem, leaf and flower,while SISRG2 was expressed at a very high level in the leaf and flower. Upon salt stress,SISRG1 was induced slowly, while SISRG2 was induced rapidly in Edkawi; however, the general trend of their expression in the untreated control was declining. These two genes are potential novel candidates for abiotic tolerance research in tomato.
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