以经过二次过滤的富营养化鱼塘养殖污水为培养液,添加外源的碳、氮、磷元索,研究了污水中不同的外源无机碳、总氮和总磷浓度对布朗葡萄藻(Botryococcus braunii)生物量、总脂和总烃含量的影响.结果表明:(1)以NaHCO3作为碳源,布朗葡萄藻的生物量和总脂含量在外源无机碳浓度为5～10 mg/L时最高,总烃含量在外源无机碳浓度为15mg/L时最高.(2)以KNO3作为氮源,布朗葡萄藻的生物量在总氮浓度为15mg/L时最高,总脂含量在总氮浓度为2mg/L时最高,总烃含量在总氮浓度为20mg/L时最高.(3)以KH2 PO4作为磷源,布朗葡萄藻生物量在总磷浓度为2mg/L时最高,总脂含量和总烃含量在总磷浓度为1.5 mng/L时最高.%Eutrophication wastewater from twice filtered fishponds was used as the culture medium, into which carbon,nitrogen,and phosphorus were added. The influences of different concentrations of inorganic carbon,total nitrogen,and total phosphorus of sewage on biomass, li-pid content,and hydrocarbon content of Botryococcus braunii were studied. The following results were reported; (1) Using NaHCO3 as the carbon source,the biomass and lipid content of B. brauniiwere the highest at the inorganic carbon concentration of 5-10 mg/L,while the highest hydrocarbon content was at the inorganic carbon concentration of 15 mg/L. (2) Using KNOg as the nitrogen source, the biomass of B. braunii was the highest at the total nitrogen concentration of 15 mg/L; the highest lipid content was at the total nitrogen concentration of 2 mg/L; the highest hydrocarbon content was at the total nitrogen concentration of 20 mg/L. (3) Using KH2PO4 as the phosphorus source,the biomass of B. braunii was the highest at a total phosphate concentration of 2 mg/L while the highest lipid content and the highest hydrocarbon content were at a total phosphorus concentration of 1.5 mg/L.
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