卵孢白僵菌 NEAU30503固态培养条件优化

摘要

Single factor screening test and response surface method were used to optimize the fermentation condi-tions in order to improve spore production of Beauveria brongniartii NEAU30503.The suitable water content,in-oculation amount,fermentation temperature and fermentation time were determined by single factor experiments. By using Box-Behnken experimental design and the response surface analytical method,the optimum fermentation conditions were obtained:the water content in solid medium was 55%;inoculation amount was 15 mL/100 g;cul-ture temperature was 27 ℃ and culture time was 7.5 d.Under these conditions,B .brongniartii NEAU30503 spor-ulation capacity reached 36.72×108 conidia/g before drying.This method is suitable for small business and facto-ries for fast spore production.%为了提高卵孢白僵菌 NEAU30503在固态培养中的产孢量，采用单因素筛选试验和响应面法对卵孢白僵菌NEAU30503固态发酵条件进行优化。在单因素试验确定最适含水量、接种量、培养温度和培养时间的基础上，应用 Box-Behnken 试验设计和响应面分析方法优化出最佳固态培养条件为：固态培养基含水量为55％，接种量为15 mL／100 g，培养温度为27℃，培养时间为7．5 d，在此条件下卵孢白僵菌 NEAU30503烘干前单位产孢量达到36．72×108孢子／g。此方法适用于小型企业和生产单位对白僵菌的快速生产。