Chelex-100法提取甘薯小象甲DNA及云南地区甘薯小象甲的分子鉴定

摘要

Sweet potato weevil (Cylas formicarius) is an important quarantine pest in the world.It not only harms sweet potato tuber but also makes wounds that induced pathogen to infect,which caused infected sweet potato to send out offensive odor and turn bitter.Accurate identification of sweet potato weevils in Yunnan,especially its genetic variation,can provide a basis for quarantine.In this research,we collected sweet potato weevils from Yuanmou County,Yunnan Province,applied a rapid method for extracting the DNA of C.formicarius by using Chelex-100 and analyzed the ITS-1 sequence of adult female and male insects.We first find that sweet potato weevils in Yuanmou County belong to the southeast subclade of the East Asia clade.This result differs from the previous report that sweet potato weevils belong to the Northeast subclade of the East Asia clade,suggesting that sweet potato weevils populations in Yuanmou County differs from those in Guangdong,Fujian,Zhejiang and Chongqing.%甘薯小象甲是国内外重要的检疫性害虫,除本身为害薯块外,引起的伤口还会诱致病菌侵入,使受害薯块发生恶臭和苦味无法食用.准确鉴定云南地区的甘薯小象甲,特别是研究其遗传变异可为检疫提供依据.本研究采集了云南元谋县甘薯小象甲,采用Chelex-100法快速提取甘薯小象甲基因组DNA,分别对雌雄成虫rDNA ITS-1序列进行系统发育分析,首次发现云南元谋县的甘薯小象甲属于东亚分支的东南亚亚支,与我国之前报道的甘薯小象甲属于东亚分支的东北亚亚支不同,说明云南元谋县的甘薯小象甲种群来源与中国广东、福建、浙江和重庆的种群来源不同.