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SSR Primer for the Identification of Sweet Potatoes and Method for Identifying Sweet Potatoes

机译:用于鉴定甘薯的SSR引物和鉴定甘薯的方法

摘要

PURPOSE: A simple sequence repeat (SSR) primer for identifying sweet potato cultivar is provided to effectively detect DNA polymorphism of the sweet potato and to be used for DNA profiling of the sweet potato. CONSTITUTION: SSR primer pairs is selected from group which consist of; a primer pair represented by sequence number 1 and 2; a primer pair represented by sequence number 3 and 4; a primer pair represented by sequence number 5 and 6; a primer pair represented by sequence number 7 and 8; a primer pair represented by sequence number 9 and 10; and a primer pair represented by sequence number 11 and 12. A DNA polymorphism detecting method of sweet potato comprises; (a) a step of extracting DNA including the SSR primer pairs from the sweet potato cultivar; (b) a step of performing PCR using the SSR primer pairs and the extracted DNA as a template; (c) a step of analyzing the PCR result.
机译:目的:提供一种用于鉴定甘薯品种的简单序列重复(SSR)引物,以有效检测甘薯的DNA多态性并将其用于甘薯的DNA分析。组成:SSR引物对选自:由序列号1和2表示的引物对;由序列号3和4表示的引物对;由序列号5和6表示的引物对;由序列号7和8表示的引物对;由序列号9和10表示的引物对;一种甘薯DNA多态性检测方法,其特征在于,所述引物对由序列号11和12表示。 (a)从甘薯品种中提取包括SSR引物对的DNA的步骤; (b)以SSR引物对和提取的DNA为模板进行PCR的步骤; (c)分析PCR结果的步骤。

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