大麻雨露脱胶真菌的分离及脱胶特性研究

摘要

Hemp dew -retting fungi was isolated from the stem of hemp in present study.GB /T18147.2 -2008 and DNS methods were used to research the separation degree of hemp bast and xylem, gum content and degummase activities of hemp by 6 dominant fungi.Results showed DMJ6 separated the hemp fiber and kept its completion.Gum content and cellulase activity of DMJ6 were the lowest,but pectinase and mannanase activities were the highest in 6 dominant fungi after 7 d degumming.Pectinase and cellulase activities of DMJ6 could reach 100 U /mL after 4 d.The relative similarity of DMJ6 and As-perigillus niger was 99% according to 18S rRNA test.%本研究从雨露沤麻后的大麻原茎上分离筛选大麻脱胶真菌。采用 GB ／T18147.2－2008和 DNS 法对6株优势真菌进行残胶率及脱胶酶活性分析。结果表明，DMJ6沤麻7 d，大麻原茎木质部及韧皮部分离较好且不破坏纤维完整性；残胶率明显低于其他；果胶酶、甘露聚糖酶活性高于其他，木聚糖酶活性较高，纤维素酶活性低于其他；此菌产酶快，4 d 果胶酶及甘露聚糖酶活性可达100 U ／mL。经18S rRNA 鉴定，DMJ6与 Asperigillus niger 相似度为99％。