黄麻耐盐性评价及盐胁迫下的生理响应

摘要

In this study,20 jute germplasm resources were cultured with hydroponics supplemented with NaCl of 250 mmol/L.8 characters of 20 jute materials were measured,including shoot height,stem diameter,upper ground weight,root weight,total weight,shoot dry weight,root dry weight and total dry weight.Then 20 jute germplasms were evaluated and classified according to salt tolerance by subordination function method and cluster analysis.3 jute materials with different salt-tolerance were treated with NaCl at different concentrations of 0 mmol/L,150 mmol/L and 250 mmol/L,and the content of soluble sugar,soluble protein and free proline were measured.The result showed significant difference of the salt tolerance among different materials.Based on the comprehensive evaluation values (D values) of 20 jute germplasm resources,they could be classified into 3 groups by cluster analysis.8 germplasms had high salt-tolerance,8 germplasms had the moderate salt-tolerance,and 4 materials were salt sensitive.The content of soluble sugar and free proline of the leaves of 3 different grades all significantly increased,but the soluble protein content significantly decreased under salt stress.When NaCl concentration was 150 mmol/L,the content of soluble sugar and free proline of the leaves of qingpi dama were significantly higher than that of tianma and nanyang changguo,and the soluble protein content were significantly higher than that in nanyang changguo.When NaCl concentration was 250 mmol/L,the content of soluble sugar,free proline and soluble protein of the leaves of tianma were significantly higher than that of nanyang changguo and qingpi dama.%试验采用水培法,对20份黄麻种质材料进行250 rnmol/L NaCl胁迫处理,通过测定株高、茎粗、水上鲜重、水下鲜重、整株鲜重、水上干重、水下干重、整株干重,利用隶属函数法、聚类分析法确定各供试材料耐盐等级,将不同耐盐等级的材料进行0、150、250 mmol/L NaCl处理,测定其可溶性糖、可溶性蛋白以及游离脯氨酸含量.结果表明:不同黄麻种质资源间耐盐性存在显著差异,根据耐盐性综合评价值D值可将其分为3类,强耐盐材料有8份,中度耐盐材料8份,盐敏感材料4份;在NaCl胁迫下,不同耐盐等级的黄麻种质材料叶片内可溶性糖和游离脯氨酸含量都显著升高,而可溶性蛋白的含量显著下降,在150 mmol/L NaCl处理下,青皮大麻叶片内可溶性糖和游离脯氨酸的含量显著高于甜麻和南阳长果,可溶性蛋白的含量显著高于南阳长果,在250 mmol/L NaCl处理下,甜麻叶片内可溶性糖、游离脯氨酸和可溶性蛋白的含量显著高于南阳长果和青皮大麻.