Genetic, Physiological and Nutritional Studies on Clostridium Strains Isolated and Screened for Characteristics Useful in Enhanced Oil Recovery, with Special Reference to High Salt Tolerance. Quarterly Report, March-May 1986

摘要

A number of experiments have been conducted involving various aspects of nutrition of clostridia that are related to practical considerations for downhole testing and use of the bacteria. Several strains of Clostridium have been used. The following experiments are discussed in this report: (1) comparisons of gas production by two strains of Clostridium, SEG-3 and Clostridium A under four different conditions of growth; (2) effect of sodium chloride on growth of Clostridium A in the basal semidefined medium and effects of casamino acids and ascorbate in NaCl-containing media; (3) response of six strains of clostridia to raffinose, FeSO sub 4 , and beet molasses, and production of emulsifying agents by these strains; (4) viability of SEG-3 and TSEG-3 cultures handled in different ways; (5) comparison of sucrose, pyruvate, and a mixture of the two as precursors of gas in the semidefined medium; and (6) effect of NaCl on gas production by resting cells. 9 figs., 4 tabs. (ERA citation 11:039676)