基于纳米金在线扫集-毛细管电泳法测定尿样中8-羟基-2′脱氧鸟嘌呤核苷

摘要

A method for the determination of 8-hydroxy-2′-deoxguanosine（8-OHdG） in urine sample by CE based on enrichment by online sweeping with nanogold was proposed.Analytical conditions were studied and following optimized parameters were given： ① moving voltage：-20 kV;② electrophoretic medium： 20 mmol·L-1 of borax solution（pH 8.2,including 10 nm nanogold solution of 200 μL and 0.1 mmol·L-1 CTMAB;③ wavelength of detection： 254 nm.It was shown that micelle was formed between nanogold and CTMAB in the electrophoretic medium,which conduced to the sweeping of 8-OHdG.The baseline resolution was attained within 10 min.Linearity range between 0.50 and 50.0 μmol·L-1 was obtained with detection limit（3S/N） of 39 nmol·L-1.The proposed method was used in the determination of 8-OHdG in a urine samples,gving recovery found by standard addition method in the range of 90.0%-104.6%.%提出了纳米金在线富集-毛细管电泳法测定尿样中8-羟基-2′脱氧鸟嘌呤核苷（8-OHdG）的方法。试验选择了以下的分析条件：①运行电压-20kV;②背景电解质为pH 8.2的20mmol.L-1硼砂（含粒径10nm纳米金溶液200μL和0.1mmol.L-1 CTMAB）;③检测波长254nm。试验证明背景电解质中的纳米金粒子与CTMAB形成胶束,提高了对8-OHdG的扫集能力,8-OHdG与dG在10min内可实现基线分离。8-OHdG浓度在0.50～50.0μmol.L-1范围内呈线性,检出限（3S/N）为39nmol.L-1。方法用于尿样中8-OHdG含量的测定,所得加标回收率在90.0%～104.6%之间。