夏凌养阴颗粒质量控制的研究

摘要

Objective To establish a quality standard for Xialing Yangyin granules. Methods Icariin of Xialing Yangyin Granules was determined by high performance liquid chromatography-evaporative light scattering detector. Radix scrophulariae, Asatragaloside Ⅳ and Flos campsis were identified by thin layer chromatography. The detemination was carried out by high performance liquid chromatography. Results Icariin showed a good hnear relationship within the range of 0. 4665-9.330 μg( r =0.9998). The average recovery was 99.47％ and RSD was 1.95 ％ ( n = 6). Conclusion This method is reliable and accurate.%目的 初步建立夏凌养阴颗粒的质量控制标准.方法 采用薄层色谱法对玄参、黄芪甲苷、凌霄花进行定性鉴别;采用蒸发光散射检测法测定黄芪甲苷的含量.结果 在薄层色谱法中可检出玄参、黄芪甲苷、凌霄花;在蒸发光散射检测法中,黄芪甲苷在0.4665～9.330μg范围内线性关系良好,r=0.9998,样品的平均回收率为99.47%,RSD为1.95%(n=6).结论 该方法简便准确,灵敏度高,重复性好.