高效液相色谱法测定枸橼酸钾溶液中枸橼酸钾的含量

摘要

Objective To develop an high performance liquid chromatography quantitative method for the determination of potassium citrate in potassium citrate solution. Methods The chromatographic conditions include column C18 (150 mm ×4.6 mm, 5 μm) and a mobile phase of 0.025 moL/L disodium hydrogen phosphate( adjusted to pH 2.5 by phosphoric acid). The flow rate was 1 mL/min and monitored at 210 nm. Results The regression equation for potassium citrate was Y= 0.3764X - 25.45, r = 0.9999 and the linear range was 429.4-1288 μg/ml. The average recovery of potassium citrate was 99.4％ and RSD was 1.3％. Conclusion This method is simple, time-saving and accurate. It can be used for routine analysis of potassium citrate in potassium citrate solution.%目的 建立高效液相色谱法测定枸橼酸钾溶液中枸橼酸钾含量的方法.方法 色谱柱采用十八烷基硅烷键合硅胶为填充剂(150mm×4.6mm,5μm),以0.025 moL/L磷酸氢二钠(用磷酸调pH值为2.5)溶液为流动相,流速:1mL/min,检测波长:210nm.结果 以峰面积(X)对进样浓度(Y)线性回归,回归方程为Y=0.3764X-25.45,r=0.9999,线性范围429.4～1288μg/ml,枸橼酸钾回收率为99.4%,RSD为1.3%.结论 本方法操作简便,测定结果准确,重复性好,可用于枸橼酸钾溶液中枸橼酸钾的含量测定.