To establish a quantitative analysis method of the triterpenoid saponins in Cy-athula officinalis. Methods: An HPLC-ELSD method was used to determine the contents of two major triterpenoid saponins in C. officinalis. The separation was performed on a Shim-pack VP-ODS column (C18, 5μm, 4.6 mm×250 mm), with a mixture solution of acetonitrile-0.1% formic acid in water (37∶63) as mobile phase. The flow rate was 1.0 mL·min-1. The drift tube temperature of ELSD detector was 105℃, the gas flow was 2.8 L·min-1. Results: The contents of 3-O-{β-D-glucopy- ranosyl-(1→2)-[α-L-rhamnopyanosyl-(1→3)]-(β-D-glucuronopyranosyl)}-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside A) and 3-O-α-L-rhamnopyranosyl-(1→3)- β-D-glucuronopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside B) were 0.47%~1.37% and 0.46%~1.32%, respectively. The linear range of were 1.0~20.0μg and 0.8~16.0μg and the average recoveries were 99.72% and 97.72%, respectively. Conclusion: The HPLC-ELSD method is accurate and suitable for the quantitative analysis of these two saponins in C. officinalis.%目的：建立川牛膝中2种主要三萜皂苷---川牛膝皂苷A、B的含量测定方法。方法：采用HPLC-ELSD法，Shim-pack VP-ODS C18（4.6 mm×250 mm，5μm）色谱柱；流动相为乙腈-0.1%甲酸水（37∶63）；体积流量为1.0 mL·min-1；ELSD漂移管温度为105℃；载气流速2.8 L· min-1。结果：3-O-{β-D-吡喃葡萄糖-（1→2）-[α-L-吡喃鼠李糖-（1→3）]-β-D-吡喃葡萄糖醛酸基}齐墩果酸-28-O-β-D-吡喃葡萄糖苷（川牛膝皂苷A）和3-O-[α-L-吡喃鼠李糖-（1→3）-β-D-吡喃葡萄糖醛酸基]齐墩果酸-28-O-β-D-吡喃葡萄糖苷（川牛膝皂苷B）的含量分别为0.47%~1.37%和0.46%~1.32%；线性范围分别为1.0~20.0μg与0.8~16.0μg；平均回收率分别为99.72%和97.72%。结论：HPLC-ELSD含量测定方法准确、简便、专属性好，适用于川牛膝的质量评价。
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