罗丹明席夫碱荧光探针的合成及在Cu2+和Fe3+检测中的应用

摘要

将罗丹明酰肼与对溴苯甲醛缩合制备了罗丹明席夫碱探针分子(RBr),并通过单晶X射线衍射和核磁共振波谱对它的结构进行了表征.实验结果表明,在体积比为1∶1的乙腈/水溶液中,RBr对Cu2+和Fe3+表现出显著的吸收和荧光选择性,光谱强度与离子浓度具有较好的线性关系.Job’s Plot实验证实RBr与Cu2+或Fe3+具有较好的配位作用,并形成1∶1的金属-探针络合物.RBr-Cu2+溶液在不同波长的紫外光照射下,吸收光谱强度明显增强.%A rhodamine schiff base fluorescent probe(RBr) was prepared by condensing rhodamine hydrazide with p-bromobenzaldehyde. Its structure was characterized by single crystal XRD and NMR spectroscopy. In aqueous acetonitrile solution,RBr exhibited significant absorbance and fluorescence selectivity for Cu2+ and Fe3+,and there was a good linear relationship between the spectral intensity and ion concentration. The Job’s Plot experiment confirmed that the spirolactam structure of RBr was induced to open upon the coordination of Cu2+/Fe3+,and a 1∶1 metal-probe complex was formed. The absorption intensity of RBr-Cu2+ solution was enhanced under the irradiation of ultraviolet light.