聚醚醚酮/纳米羟基磷灰石生物材料对MG-63细胞增殖作用

摘要

Objective To prepare PEEK/HA composite materials and,study MG-63 cells Proliferation effect of the composite materials at different periods and under different concentrations and Determine MG-63 cell adhesion rate of the material.Method Based on the polycondensation of polyether-ether-ketone,adding 10％,20％ hydroxyapatite blending into PEEK,to prepare biological composite materials.Using Inverted fluorescence microscope to observe cell morphology MTT method was used to study the cell proliferation in various conditions.Results The materials cytotoxicity level at 0 or Ⅰ,which indicates that PEEK/HA composites are non-toxic to MG-63 cells.In a concentration of 8 mg/ml,the material has an obvious role in cell proliferation.In a concentration of 64 mg/ml concentration,the effect of PEEK composites on cell proliferation is minimum.Cell adhesion rate increases with an increase of HA content in PEEK/HA material,HA can improve cell adhesion to the materials.Conclusion PEEK/HA composites have no obvious toxicity to MG-63 cells.%目的 制备PEEK/HA复合材料,研究在不同时间和不同浓度条件下对MG-63细胞增殖的作用,评价其细胞毒性.测定该材料对MG-63细胞黏附率.方法 合成聚醚醚酮,与一定含量的纳米羟基磷灰石共混,制备PEEK/HA复合材料.通过倒置荧光显微镜观察材料对细胞形态影响,采用MTT法研究在不同时间和不同浓度条件下对细胞增殖的作用,评价其细胞毒性.结果 PEEK/10％HA、PEEK/20％HA对细胞形态影响显示,都有大量活细胞,PEEK/20％HA中活细胞多于PEEK/10％HA,说明PEEK/20％HA对细胞增殖作用比PEEK/10％HA强,均有黏附现象.各组材料细胞毒性级别均在0或Ⅰ级,说明各组材料对细胞无毒性.在8mg/ml浓度下材料对细胞增殖有明显促进作用,随着浓度的增加,对细胞生长有明显的抑制作用,随着浓度进一步增加,材料对细胞增殖作用逐渐减小,曲线呈下降趋势,当浓度为64mg/ml时,PEEK复合材料对细胞增殖作用最小.PEEK/HA复合材料随着HA含量的增加,细胞黏附率增加,HA可以改善材料黏附性能.结论 PEEK/HA材料对MG-63细胞无毒性.