维生素C对大鼠实验性根尖周炎影响的初步研究

摘要

Objective:To explore the effect of vitamin C on the development of the induced rat periapical periodontitis.Methods:Periapical disease was induced by pulp exposure of the mandibular first molar on 100 male Wistar rats (8-week-old).Rats were divided into vitamin C groups (0,0.1,0.5 and 1 mg/mL,respectively)and a normal group,then were sacrificed on day1,3,7,14 and 21.We measured the area of the periapical lesions by radiographs and obtain histological results by HE staining.Results:Periapical lesions were significantly expanded from day 3 onwards,in a time-dependent manner,and peaked on day 21.There was no difference between day 1 and 3.On day 7,14 and 21,the periapical lesion areas of practiced groups were remarkably lower than that of the control group. The periapical lesions of the 0.1 mg/mL group was the lowest among all practiced groups.Vitamin C groups also had less bone resorp-tion lacunae and smaller alveolar bone destruction.Conclusions:To some extent,vitamin C effectively inhibited the progress of the rat periapical periodontitis.%目的：探讨维生素 C 对大鼠实验性根尖周炎形成过程的影响。方法：选用100只8周龄 Wistar雄性大鼠，！腔直接暴露法建立根尖周炎模型，将大鼠分成维生素 C 干预组（0．1、0．5、1．0 mg/mL 维生素 C 灌胃）及对照组，术后1、3、7、14、21 d处死，通过 X线测量法和 HE 染色观察根尖周病变的大小和组织学变化。结果：维生素 C 干预组和对照组根尖透射区的面积从3 d开始增大，呈时间依赖性，21 d时透射面积到达高峰。术后7、14、21 d维生素C 干预组的根尖透射区的面积均小于对照组，差异具有统计学意义（P＜0．05），其中0．1 mg/mL组根尖透射区的面积明显小于其它各组（P＜0．05）。术后7、14、21 d 维生素 C 干预各组骨吸收陷窝数及牙槽骨破坏范围均小于对照组。结论：全身应用维生素 C 对抑制大鼠根尖周炎的进展有一定的作用。