异种脱细胞真皮基质对新生血管作用的研究

摘要

目的：探讨不同异种脱细胞真皮基质对内皮细胞血管生成的促进作用。方法：利用人脐静脉内皮细胞（ HUVEC），与猪脱细胞真皮基质（ P⁃ADM）和牛脱细胞真皮基质（ B⁃ADM）共培养，以Bio⁃Gide可吸收生物膜和空白组作对照，ELISA法分别检测细胞接种4、6、12、24、48、96 h共培物上清中血管内皮生长因子（ VEGF）的含量；GFP慢病毒转染于HUVEC后，在倒置相差显微镜下计数共培物上细胞管型的数目。结果：在细胞接种24 h时，ADM组与Bio⁃Gide组和空白组比较VEGF的浓度更高（ P＜0．05）；48 h时P⁃ADM组VEGF的浓度高于与Bio⁃Gide组和空白组（ P＜0．05）。转染GFP慢病毒的HUVEC与生物膜共培养6～48 h时，B⁃ADM组细胞管型多于P⁃ADM组和Bio⁃Gide组；24 h内，P⁃ADM组多于Bio⁃Gide组；空白组未见明显管型。结论：P⁃ADM与B⁃ADM比Bio⁃Gide具有更好的成血管作用，且B⁃ADM效果更明显，提示异种ADM有较好的促新生血管的作用，可作为替代物应用于膜龈手术。%Objective:To research the effect of xeno⁃acellular dermal matrix(xeno⁃ADM) on vasculogenesis of human umbilical vein endothelial cell( HUVEC) . Methods:Porcine acellular dermal matrix ( P⁃ADM) and bovine acellular dermal matrix ( B⁃ADM) were co⁃cultured with HUVEC, using blank and Medical collagen membrane( Bio⁃Gide) as control. The concentration of vascular endo⁃thelial growth factor ( VEGF) collected from different groups at 4, 6, 12, 24, 48, 96 h was determined by ELISA test. Meanwhile, the number of cellular casts were counted by observing HUVEC infected by GFP expression lentivirus. Results:The VEGF concentration of xeno⁃ADM groups were higher than that of the control groups( P<0.05) after 24 h, and P⁃ADM was more than that of the control groups after 48 h(P<0.05).The number of cellular casts of P⁃ADM group was about as twice as that of the control group after 24 h. The B⁃ADM group improved less than the P⁃ADM group. No cellular cast was found in the control group. Conclusions:Xeno⁃ADM has better effect on vasculogenesis than traditional medical collagen membrane, which suggesting that it has the potential to be the next generation of surgical material applying to mucogingival therapy.