光阱位置操纵系统的研究

摘要

An optical tweezers, which can be used to mainipulate biological sample in three dimensions, was incorporated into a LEICA DMIRBE inverted microscope with bottom port. During setting up this system, transverse position mainpulating system of an optical trap was studied especially. Theoretical analysis and experimental results indicate that the transvers position of an optical trap can be changed by laterally moving or rotating a mirror. The mechanisms of optical trap steering systems by moving and rotating mirrors were explained in detail, resp ectively. In addition, comparing with moving mirror system the rotating mirror system is of benefit to increase the mainipulating speed of an optical trap, simp lify the mechanism of an optical tweezers, reduce its cost, and shorten the cycle of completing an optical tweezers.%利用He-Ne激光器,在LEICA DMIRBE倒置显微镜上建立了可对生物样品进行三维操纵的光镊系统。在光镊的研制过程中,我们重点对光阱的横向位置操纵系统进行了研究。理论分析及 实验表明,利用平移反射镜及转动反射镜的方案均可实现光阱位置的横向操纵。本文对平移 反射镜及转动反射镜实现光阱位置操纵的机理进行了详细的论述,并对操纵过程中保证光阱 质量不发生变化的条件进行了理论分析和说明。此外,比较分析表明,与平移反射镜系统相比 ,转镜系统在光镊技术中的应用可加快光镊操纵速度、简化光阱位置操纵系统的复杂程度、 有利于系统开发成本的减少及开发周期的缩短。