非小细胞肺癌中TRIM29、caspase-8、β-catenin蛋白表达情况及临床意义研究

摘要

目的 探讨TRIM29、caspase-8和β-catenin在非小细胞肺癌(NSCLC)组织中的表达及其临床意义.方法 分别通过免疫组织化学法检测254例NSCLC患者肺癌组织(NSCLC组)及其癌旁正常肺组织(对照组)中TRIM29、caspase-8和β-catenin表达情况,并分析其与患者临床特征的关系.结果 NSCLC组中TRIM29的阳性表达率和β-catenin的异常表达率均明显高于对照组,caspase-8的阳性表达率明显低于对照组(P<0.01);肺鳞癌组织中TRIM29阳性表达率高于肺腺癌组织,β-catenin的异常表达率低于肺腺癌组织,而caspase-8在两种组织中的表达无明显差异;TRIM29、caspase-8阳性表达患者的平均生存时间明显低于阴性表达者,β-catenin高表达患者的平均生存时间明显低于低表达患者(P<0.01);Cox多因素分析显示,淋巴结转移、肿瘤分期、TRIM29、caspase-8和β-catenin表达水平是影响患者生存的独立预后因素.结论 TRIM29、caspase-8、β-catenin在NSCLC中均有异常表达,同时与NSCLC患者淋巴结转移、病理分类、临床分期及患者生存期有密切关系,可以作为临床中判断NSCLC患者病理分类、分期及预后的参考依据.%Objective To explore the expression of TRIM29, caspase-8 andβ-catenin in non-small-cell lung cancer (NSCLC) and their clinical significance. Method Immunohistoehemical assay was used to detect the gene expression of TRIM29, caspase-8 and β-catenin in tumor tissues (NSCLC group) and adjacent normal tissues (control group) of 254 NSCLC patients, and the correlation with patient's clinicopathological features was analyzed. Result The positive ex-pression rate of TRIM29 and abnormal expression rate ofβ-catenin in NSCLC tissues were significantly higher than that in control group, but the study group had lower expression rate of caspase-8 than control group (P<0.01);TRIM29 ex-pression in lung squamous carcinoma was higher than that in lung adenocarcinoma and the abnormal expression rate ofβ-catenin was lower in lung squamous carcinoma, while the caspase-8 expression in these two tissues was of no significant difference;Survival time of patients with positive TRIM29 and caspase-8 expressions was significantly lower than that of negative patients, and survival time of patients withβ-catenin expression was significantly lower than that of low-expres-sion patients (P<0.01). Cox multi-variate analysis showed that lymph node metastasis, tumor stage, TRIM29, caspase-8 and β-catenin expression levels were independent prognostic factors influencing the survival of patients. Conclusion The expression of TRIM29, caspase-8 and β-catenin were abnormal in NSCLC, and is closely correlated with NSCLC lymph node metastasis, pathological classification, clinical stage, as well as the patients'survival time, which can be used as references for pathological classification, clinical staging and prognosis of patients with NSCLC.