目的 探讨INSR基因多态性与卵巢上皮癌(EOC)铂类化疗敏感性的关系.方法 收集339例接受手术及术后化疗的EOC患者资料,INSR基因标签的单核苷酸多态从HapMap基因组数据结合现有文献中筛选出来,胰岛素受体基因型和等位基因频率分布由ABI3100-Avant测序.比较EOC化疗敏感与不敏感患者中INSR基因多态性的分布差异及可能影响EOC化疗敏感性的基因型.结果 与rs2252673 GC+CC基因型相比,rs2252673 GG基因型携带者的铂类药物化疗敏感性更低;与rs3745546 GG+GC基因型相比,rs3745546 CC基因型携带者的铂类药物化疗敏感性更低.Logistic分析显示rs2252673和rs3745546基因多态性是影响EOC患者化疗敏感性的主要因素(P﹤0.05).结论 INSR rs2252673和rs3745546的基因多态性可能与EOC患者铂类化疗敏感性有关.%Objective To investigate the influence of insulin receptor (INSR) gene polymorphism in platinum-based chemotherapy response in epithelial ovarian cancer (EOC). Method A total of 339 patients who underwent post-operative chemotherapy were recruited. Tag single nucleotide polymorphism (tagSNP) of INSR gene was screened based on HapMap and available literature. Frequency distribution of the genotypes and alleles in INSR gene was se-quenced by ABI3100-Avant to compare the distribution of INSR gene polymorphism between chemosensitive group and chemoresistant group and to identify the genotype which might influence chemosensitivity in EOC patients. Result Compared with GC+CC genotype of rs2252673, GG of rs2252673 showed decreased platinum-based chemosensitivity in INSR gene;and compared with GG+GC genotype of rs3745546, CC of rs3745546 showed less chemosensitivity to platinum medications. Logistic analysis showed that the polymorphism of rs2252673 and rs3745546 were the main fac-tors influencing the chemosensitivity of EOC (P<0.05). Conclusion The polymorphisms of rs2252673 and rs3745546 of INSR gene may be associated with platinum-based chemosensitivity of EOC patients.
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