微波法与超声波法比较研究铁棒锤的挥发油

摘要

目的 研究铁棒锤微波和超声波2种不同提取方法 提取挥发油成分的异同.方法 采用微波辅助提取法和超声波辅助提取法提取铁棒锤中的挥发油.采用GC-MS结合计算机检索对所得挥发油成分进行分析和鉴定,用峰面积归一法测定各组分的相对含量.结果 分别用2种方法 从铁棒锤中提取挥发油,鉴定出30和33种化合物.结论 2种不同方法 提取铁棒锤挥发油化学组成成分差异性较大,部分挥发油成分还具有毒性,这些挥发油成分是否是铁棒锤毒性成分的组成部分,还有待进一步研究.%Objective To study the difference in chemical constituents between the 2 kinds of extraction methods (microwave-assisted and ultrasound-assisted)from Radix Aconiti pendulum.Methods By means of microwave-assisted extraction and ultrasound-as-sisted extraction,volatile oils were extracted from Radix Aconiti pendulum.The chemical compositions were analyzed and identi-fied by GC-MS and the relative contents of the constituents were calculated by area normalization method.Results 30 and 33 kinds of volatile components were identified respectively by the 2 methods used to extract volatile oil from Radix Aconiti pendulum. Conclusion The volatile oil′s chemical composition extracted by these 2 different methods are significantly different,and part of the chemical compositions was toxic to humans.However,whether the toxic volatile oil compositions are parts of the toxic composi-tion of Radix Aconiti pendulum or not still needs to be further studied.