Objective To study the principal active components and to determine the 7 kinds of steroidal saponins of Rhizoma Paridis plants from different sources and establish a reference for the identification and quality evaluation.Methods Polyphyllin Ⅶ, polyphyllin H,polyphyllin Ⅵ,polyphyllin Ⅱ,polyphyllinⅠ,polyphyllin Ⅴ and dioscin of 15 batches of Rhizoma Paridis plants from different sources were analyzed by HPLC on an Inertsil ODS-3C18(150mm×4.6mm,5μm) column.The mobile phase was composed of acetonitrile-water(50:50) at a flow rate of 1.0 mL·min-1.The column temperature was 35 ℃.Accordingly,the detection wavelength was set at 203 nm and the injection volume was 20.0μL.Results In the samples,the total content of polyphyllin Ⅶ,polyphyllin Ⅵ,polyphyllin Ⅱ and polyphyllin Ⅰ was between 1.849-20.222 mg·g-1,and the total content of polyphyllin Ⅶ,polyphyllin H,polyphyllin Ⅵ,polyphyllin Ⅱ,polyphyllin Ⅰ,polyphyllin Ⅴand dioscin was between 2.351-37.366 mg·g-1 .There sults showed this method had high precision,good stability and repeatability (RSD≤3.0%),and the averagere-coveries were 95.92%-103.20% (RSD≤3.0%,n=5).Conclusion The results showed that the difference of polyphyllins content of Rhizoma Paridis plants varied from different producing areas and variety classes.HPLC fingerprints of the 15 batches of Rhi-zoma Paridis plants were established,and could be used for the distinguishing the authenticity,controlling and determing the Rhi-zoma Paridis.%目的 分析不同来源重楼属植物的主要活性成分,比较7种甾体皂苷的含量,为重楼药材的鉴别和品质评价提供参考依据.方法 采用HPLC法测定不同来源重楼属15批药材中重楼皂苷Ⅶ、重楼皂苷H、重楼皂苷Ⅵ、重楼皂苷Ⅱ、薯蓣皂苷、重楼皂苷Ⅰ和重楼皂苷Ⅴ的含量.色谱条件为InertsilODS-3C18色谱柱(150 mm×4.6 mm,5μm);流动相为乙腈-水(50:50);检测波长为203nm;流速为1.0mL·min-1;柱温为35℃;进样量为20.0μL.结果 15批样品中,重楼皂苷Ⅶ、重楼皂苷Ⅵ、重楼皂苷Ⅱ和重楼皂苷Ⅰ4种皂苷含量之和均在1.849~20.222mg·g-1之间;样品中7种皂苷含量之和在2.351~37.366mg·g-1之间.精密度、稳定性和重复性实验的RSD值均≤3.0%,7种皂苷的平均加样回收率均在95.92%~103.20%之间(RSD≤3.0%,n=5).结论 因产地或种类不同,重楼皂苷的含量存在一定差异.基于15批重楼样品的HPLC图谱,构建了重楼药材的HPLC指纹图谱,实验结果 可用于重楼的真伪鉴别和质量评价.
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