Flavanones (hesperidin, naringenin, naringin, and poncirin) in industrial, hand-squeezed orange juices and from fresh-in-squeeze machines orange juices were determined by HPLC/DAD analysis using a previously described liquid-liquid extraction method. Method validation including the accuracy was performed by using recovery tests. Samples (36) collected from different Brazilian locations and brands were analyzed. Concentrations were determined using an external standard curve. The limits of detection (LOD) and the limits of quantification (LOQ) calculated were 0.0037, 1.87, 0.0147, and 0.0066 mg 100 g−1 and 0.0089, 7.84, 0.0302, and 0.0200 mg 100 g−1 for naringin, hesperidin, poncirin, and naringenin, respectively. The results demonstrated that hesperidin was present at the highest concentration levels, especially in the industrial orange juices. Its average content and concentration range were 69.85 and 18.80–139.00 mg 100 g−1. The other flavanones showed the lowest concentration levels. The average contents and concentration ranges found were 0.019, 0.01–0.30, and 0.12 and 0.1–0.17, 0.13, and 0.01–0.36 mg 100 g−1, respectively. The results were also evaluated using the principal component analysis (PCA) multivariate analysis technique which showed that poncirin, naringenin, and naringin were the principal elements that contributed to the variability in the sample concentrations.
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机译：使用先前描述的液-液萃取方法，通过HPLC / DAD分析测定工业，手压橙汁和鲜榨机中橙汁中的黄酮类（橙皮苷，柚皮苷，柚皮苷和poncirin）。包括准确性在内的方法验证是通过使用恢复测试进行的。分析了从巴西不同地区和品牌收集的样本（36）。使用外标曲线确定浓度。计算的检出限（LOD）和定量限（LOQ）为0.0037、1.87、0.0147和0.0066 0.00mg 100 g -1 和0.0089、7.84、0.0302和0.0200 mg 100 g < sup> -1 分别用于柚皮苷，橙皮苷，poncirin和柚皮苷。结果表明橙皮苷的含量最高，特别是在工业橙汁中。平均含量和浓度范围为69.85和18.80-139.00 mg 100 g -1 。其他黄烷酮的浓度最低。所发现的平均含量和浓度范围分别为0.019、0.01-0.30和0.12，以及0.1-0.17、0.13和0.01-0.36mg100μg -1 。还使用主成分分析（PCA）多元分析技术对结果进行了评估，该技术表明poncirin，naringenin和naringin是影响样品浓度变化的主要元素。
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