鹰嘴豆多糖的分离纯化及其抗氧化作用研究

摘要

Objective To isolate and purify the homogeneous polysaccharides from Cicer arietinum and to evaluate the antioxidant ac-tivity .Methods After the crude polysaccharides were obtained from the fruits of Cicer arietinum by water extraction ,the polysac-charides were purified by DEAE-Cellulose 52 and Sephacryl S-300 to give the homogenous polysaccharide .High performance gel permeation chromatography (HPGPC) proved that the polysaccharides were homogenous .The carbohydrate content of polysac-charide was measured by phenol-sulfuric acid method .The monosaccharide composition of the homogenous polysaccharide was ana-lyzed by GC and GC-MS after hydrolysis .The antioxidant activity of the homogenous polysaccharide was measured by DPPH · scavenging .Results The results showed that the polysaccharides (CA-1b) isolated from Cicer arietinum was homogeneous with relative molecular weight of 1 .33 × 106 Da .CA-1b was made up of Ara ,Man ,Glc and Gal .And their ratio was 2 .2:3 .1:6 .2:1.0 .DPPH · scavenging experiments showed that the CA-1b had a good antioxidant activity and there was a certain relationship between the activity and concentration with IC5018 .2 μg · mL -1 .Conclusion These results provide a theoretical basis for further research and development of Cicer arietinum .%目的 对鹰嘴豆果实多糖进行分离纯化得到均一多糖,对分离得到的均一多糖的抗氧化活性进行研究.方法 利用热水对鹰嘴豆多糖进行提取,乙醇沉淀得到粗多糖;粗多糖经DEAE-Cellulose 52和Sephacryl S-300柱层析纯化,得到均一多糖CA-1b;高效凝胶渗透色谱法(HPGPC)验证CA-1b纯度和相对分子质量;酸水解、GC-MS对CA-1b进行糖组分分析;DPPH·清除评价均一多糖的体外抗氧化活性.结果 鹰嘴豆果实中分离得到的多糖CA-1b为均一多糖,相对分子质量为1.33×106 Da,由阿拉伯糖(Ara)、甘露糖(Man)、葡萄糖(Glc)和半乳糖(Gal)组成,其摩尔比为2.2:3.1:6.2:1.0.DPPH·清除实验表明,CA-1b具有良好的抗氧化活性,且呈现一定的量效关系,半数清除质量浓度IC50为18.2μg·mL-1.结论 研究结果可为鹰嘴豆的深加工和进一步研究开发奠定理论基础.