SM22琢启动子靶向性调控血管平滑肌细胞过表达 p27的研究

摘要

ObjectiveTo construct Lenti-SM22alpha-p27-EGFP recombinant lentiviral vectors targeting vascular smooth muscle cells (VSMCs) and to investigate the impact on VSMCs proliferation by cell cycle analysis. Methods: Recombinant lentiviruses were constructed. The viruses were transfected into VSMCs and vascular endothelial cells (VECs). Results: SM22α-p27 lentiviruses exhibited effective and specific inhibition on VSMCs. SM22α promoter targeted p27 was able to be selectively over-expressed in VSMCs and to inhibit proliferation by cell cycle arrest at G0/G1. Conclusion: Recombinant lentiviruses driven by SM22α promoter could selectively infect VSMCs, thus lead to p27 protein over-expression and G0/G1 arrest.%目的：构建由 SM22α特异性启动子介导血管平滑肌细胞（VSMCs）靶向性过表达 p27蛋白的慢病毒载体来研究对 VSMCs 细胞周期的影响。方法：构建重组慢病毒载体（Lenti-SM22α-p27-EGFP），感染原代培养的 VSMCs 及内皮细胞（VECs）。结果：SM22α-p27慢病毒能对 VSMCs 表现出高效且精确的抑制作用。SM22α启动子靶向标记的 p27能在 VSMCs 内过表达，且通过 G0/G1期阻滞来抑制细胞增殖。结论：由SM22α特异性启动子驱动的重组慢病毒载体能选择性感染 VSMCs，有效地引起 p27蛋白过表达并产生G0/G1期阻滞。