青藏高原红景天属植物药材中五种有效成分HPLC分析

摘要

In order to evaluate the differences and similarities between the primary constituents in Rhodiola L.genus,an improved liquid chromatography method coupled with diode array detector was developed for the simultaneous determination of Salidroside,tyrosol,gallic acid,catechin and epicatechin in fourteen medicinal plants belonging to the Rhodiola L.genus in Qinghai-Tibetan Plateau.A column of Phenomenex Luna C18 (250 × 4.6 mm,5μm)was used.The mobile phase consisted of acetonitrile and water with gradient elution.The flow rate was 1.0 mL/min with UV detection wave length at 276 nm.The column temperature was 30 ℃.The experimental results show that the five bioactive compounds were separated perfectly under the conditions of determination.Their recoveries are over 97.36％ and the RSDs are less than 2.31％.this method was sample,accurate and sensitive,so it can be used for the quality control of Rhodiola.:%为了比较红景天属不同红景天药材的有效成分的差异,采用HPLC-DAD法建立了同时测定青藏高原14种红景天属药材中红景天苷、酪醇、没食子酸、儿茶素、表儿茶素含量的方法.色谱柱为Phenomenex Luna C18(250×4.6 mm,5μm)；以甲醇-水为流动相,梯度洗脱；流速为1.0 mL/min;检测波长为276 nm;柱温为30℃.结果表明,在五种化合物在选定的条件下能得到较好的分离,线性关系良好.方法回收率在97.36％以上,RSD小于2.31％.该方法简单快速,可为红景天属药材的质量控制提供科学依据.