8种五加属植物根皮中五加酸和贝壳烯酸的RP-HPLC法定量分析

摘要

A RP-HPLC method for quantitative analysis of acanthoic acid and kaurenoic acid was established. The condition of HPLC was optimized. Acanthoic acid and kaurenoic acid from root barks of eight kinds of Acanthopanax Miq. plants were quantitative analysed by the methodology test. The best condition is established as follows: the separation is performed with a ODS-C_(18) column (250 mm×4.6 mm, 5 μm) at 30 ℃, and the mobile phase is CH_3CN-H_2O (CH_3CN 10%→100% at 0-60 min, CH_3CN 100% at 60-70 min). The acanthoic acid and kaurenoic acid are detected at 207 nm, the flow rate is 1 mL/min. The liner range of acanthoic acid and kaurenoic acid are 0.107 0-0.535 1 g/L and 0.093 0-0.464 9 g/L, respectively, and give correlations (r) of 0.999 9 and 0.999 8. The recoveries of acanthoic acid ad kaurenoic acid are 97.93%(standard deviation is 1.33%) and 98.12%( standard deviation is 1.16%), respectively.%建立反相高效液相色谱法(RP-HPLC)测定五加酸和贝壳烯酸的定量分析方法,利用最优色谱条件对8种中韩五加属植物根皮中的五加酸和贝壳烯酸进行定量分析.研究结果表明,最佳色谱条件为:ODS-C_(18)色谱柱(250 mm×4.6 mm,5 μm);流动相:乙腈-水(0～60 min时乙腈含量为10%→100%,60～70 min时乙腈含量为100%);检测波长为207 nm;柱温为30 ℃;流速为1.0 mL/min.五加酸和贝壳烯酸线性范围分别为:0.107 0～0.535 1 g/L (相关系数r=0.999 9)和0.093 0～0.464 9 g/L(r=0.999 8),加样回收率分别为97.93%(标准偏差为1.33%)和98.12%(标准偏差为1.16%).