探讨白芍总苷在正常大鼠体内的组织分布特点,为预测其药理作用及不良反应提供依据.正常大鼠按2.82 g/kg灌胃给予TGP药液后1、3、6h取心、肝、脾、肺、肾、胃、小肠、大肠等组织,各组织匀浆后,将匀浆液制成冻干粉,HPLC法测定冻干粉中芍药苷和芍药内酯苷浓度,计算各组织中两者浓度.结果显示1h各组织中均能测到芍药苷和芍药内酯苷,3h除胃和小肠外,其他各组织中两者浓度均达到最大值,小肠、胃、大肠及肾、脾、肝中浓度较高,6h小肠、大肠、胃中浓度较高,其他各组织中浓度较低.说明灌胃TGP后组织分布迅速且广泛,胃、小肠、大肠及肾、脾、肝是主要分布器官,容易在胃肠蓄积,其他组织中蓄积较少,为进一步研究白芍总苷的药理作用及作用机理提供了指导,同时为白芍归经理论提供了一定的现代科学依据.%This paper investigated tissue distribution features of total glucosides of paeony (TGP) in normal rats for providing evidence to predict pharmacologic actions and adverse reaction. Heart, liver, spleen, lung, kidney, stomach, small intestine and large intestine were gotten from normal rats after an oral administration of 2. 82 g/kg TGP 1 h,3 h and 6 h. Homogenate all tissues. Make homogenate into freeze-dry powder, the concentration of paeoniflorin (Pae) and albiflorin (Alb) in which was determined with HPLC. The concentration of the two in all tissues was calculated. 1 h Pae and Alb could be checked out in all tissues. 3 h,except stomach and small intestine,the concentration of Pae and Alb in other tissues arrived the maximum, which was higher in small intestine, stomach, large intestine, kidney, spleen and liver. 6 h the concentration of Pae and Alb in small intestine,large intestine and stomach was higher, other tissues lower. All results demonstrated,after oral administration of TGP, the drug was distributed rapidly and widely in tissues. Small intestine, stomach, large intestine and kidney, spleen, liver were the chief distribution tissues and TGP readily accumulated in stomach and intestines, less in other tissues, which provides guidance for further study of the pharmacological actions and mechanism of TGP,and meanwhile provides some scientific evidences for channel entry theory of white peony root.
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