龙柏乙酸乙酯萃取物抑菌活性成分的分离与鉴定

摘要

采用活性追踪法和柱层析法,以苹果腐烂病菌、葡萄白腐病菌等作为供试菌种,从龙柏乙醇提取物的乙酸乙酯萃取物中分离得到抑菌活性成分.结果表明,在供试浓度为2 mg/mL时,龙柏乙酸乙酯萃取物对6种植物病菌均有一定的抑制作用,其中对苹果腐烂病菌和葡萄白腐病菌抑菌活性最好,抑菌率分别为83.06％和78.87％.其活性成份桧脂素对苹果腐烂病菌、葡萄白腐病菌、小麦赤霉病菌、葡萄黑痘病菌、苹果轮纹病菌和黄瓜枯萎病菌的EC50分别为0.38、0.18、0.47、0.59、0.70 mg/mL和1.94 mg/mL.%Antifungal components,which isolated from ethanol extract of Juniperus chinensis cv. Kaizuca with ethyl acetate, were tested against Valsa mali,Coniothyrium diplodiella, and so on .based on bioassay-guided method. The results showed that ethyl acetate extracts of J. chinensis had certain inhibition effects to all tested fungi, and the best inhibition rates against V. mali and C. diplodiella were 83. 06% and 78. 87% at 2 mg/mL, respectively. The EC50 of the active component Savinin against V. mali,C. diplodiella,Gibberella zeae,Elsinoe ampelina,Physalospora piricola and Fusarium oxysporum were 0.38,0.18,0.47,0.59,0.70 mg/mL,and 1.94 mg/mL,respectively.