The aim of this study was to determine four naphthoquinones in roots of Arnebia decumbens from ten different origins by HPLC and a comparison was made for the difference of contents in different origins.Acetyl shikonin,isobutyryl shikonin,deoxyshikonin and 2-methylbutyryl shikonin were used as reference substances.Agilent-C18 (250 mm × 4.6 mm,5 pm) was used for chromatographic separation.The mobile phase was acetonitrile-0.05％ formic acid solution (70 ∶ 30) with isocratic elution.The flow rate was 1 mL/min,the detection wavelength was 275 nm and the column temperature was 30 ℃.The four components in A.decumbens can be separated well,and the linearity,repeatability and recovery were obtained.There were differences in the contents of four components in 10 species.It may be related to the species and harvesting time of the plant.Cause of the high contents of acetyl shikonin and 2-methylbutyryl shikonin in A.decumbens,it was suggested that A.decumbens can be used as an alternative resource for natural anti-tumor components and their derivatives,so as to provide reference for the exploitation and utilization of medicinal materials.%采用HPLC测定10个产地硬萼软紫草根中4种萘醌类成分含量,比较其差异.以乙酰紫草素、去氧紫草素、异丁酰紫草素、(2-甲基正丁酰基)紫草素为对照品,采用Agilent-C18(250 mm×4.6 mm,5μm),以乙腈-0.05％甲酸水溶液(70∶ 30)为流动相,1 mL/min,检测波长275 nm,柱温30℃.4种成分分离良好,线性、重复性以及回收率均符合要求.不同产地的样品中4种化学成分含量存在差异,可能与植物的生境及采收时间有关,活性成分乙酰紫草素、(2-甲基正丁酰基)紫草素在各样品中含量较高,推断其可作为天然抗肿瘤成分及其衍生物的备选资源,为其资源开发利用提供参考依据.
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