葡萄籽原花青素通过Nrf2/ARE信号通路抗高糖诱导的HUVEC-12细胞氧化损伤

摘要

研究葡萄籽原花青素提取物(GSPE)对高糖诱导的人脐静脉内皮细胞HUVEC-12氧化应激损伤的保护作用及其相关机制.建立高糖诱导的HUVEC-12细胞模型,测定细胞活力,检测细胞内活性氧(ROS)水平、乳酸脱氢酶(LDH)与超氧化物歧化酶(SOD)活性及Nrf2/ARE信号通路中相关基因mRNA水平和蛋白含量.结果显示GSPE作用后显著提高HUVEC-12细胞活力,抑制高糖诱导的细胞内ROS水平升高,增强SOD活性(P＜0.05),并呈现剂量依赖效应.GSPE作用能同时提高抗氧化转录因子Nrf2和下游区GSH-Px、HO-1、γ-GCS、NQO1基因的表达量以及HO-1、NQO1蛋白的含量(P＜0.05).结果表明GSPE能通过激活Nrf2/ARE通路对抗高糖诱导的HUVEC-12细胞氧化应激损伤.%The objective of this study is to explore the protective effect and mechanisms of grape seed proanthocyanidin extract (GSPE) against oxidative stress of HUVEC-12 cells induced by high glucose.HUVEC-12 cell model induced by high glucose was established.The proliferation of HUVEC-12 cells were evaluated.The levels of reactive oxygen species (ROS),lactate dehydrogenase (LDH) and superoxide superoxide dismutase (SOD) were assayed.Both mRNA expression levels and protein expression levels of Nrf2/ARE signaling pathway related genes were detected.The results showed that GSPE intervention can improve the antioxidant capacity of HUVEC-12 cells significantly,decrease the ROS levels in cells induced by high glucose,improve the SOD activity and the cell vitality in a dose-dependent effect (P ＜ 0.05).The analysis showed that GSPE can also increase the mRNA expression levels of Nrf2,GSH-Px,HO-1,γ-GCS,NQO1 and protein contents of HO-1,NQO1.The results indicated that GSPE may protect against the oxidative damage of HUVEC12 cells induced by high glucose via the activation of Nrf2/ARE signal pathway.