目的 应用表面增强激光解析电离飞行时间质谱(SELDI-TOF-MS)技术检测新疆哈萨克族食管癌患者血清蛋白质表达谱,建立其诊断模型.方法 采用弱阳离子交换蛋白芯片(CM10)及SELDI-TOF-MS技术,选取2008年1至8月新疆医科大学第一附属医院胸外科41例新疆哈萨克族食管癌患者和20名哈萨克族正常对照的血清蛋白表达谱进行检测,通过ZUCI-Protein Chip Data Analyze System软件包分析所得到的数据.用留一法交叉验证评估模型的判别效果.结果 筛选出7个质荷比(m/z)位于6900.3285,13 790.9241,8790.8130,15 964.6951,16 152.0872,4488.4818,4979.4223的蛋白标记物,构建新疆哈萨克族食管癌诊断模型.经留一法交叉验证,该诊断模型敏感度为100%(41/41),特异度为100%(20/20).结论 SELDI-TOF-MS技术是食管癌临床血清学诊断的一个新方法.应用其所建立的食管癌诊断模型灵敏度和特异性较高.对新疆食管癌高发民族哈萨克族患者的临床早期诊断提供了新的血清学诊断方法.%Objective To detect the serum protein profiles of Kazakh's esophageal carcinoma (EC)patients in Xinjiang by SELDI-TOF-MS ( surface enhanced laser desorption & ionization time-of-flight mass spectrometry) and build up a diagnostic model of Kazakh's EC in Xinjiang. Methods The serum samples from 41 Kazakh's EC patients and 20 Kazakh's healthy controls were collected and analyzed on weak cation exchange and hydrophobic surface protein chip by SELDI-TOF-MS technology. The differentially expressed markers of esophageal carcinoma were detected. Results The values of M/Zs were significantly different between Kazakh's EC patients and controls (P ＜ 0. 05 ). Among these, 6 proteins peaks were up-regulated (5495. 2265, 15 964. 6951, 16 152. 0872, 4488. 4818, 8164. 7652, 4979. 4223 ) and 4 down-regulated(6900. 3285, 13 790. 9241, 8790. 8130, 8714. 7915 ) in the Kazakh' s EC group. According to cross validation, the model of Kazakh's EC made up of 7 proteins ( M/Z 6900. 3285, 13 790. 9241,8790. 8130,15 964. 6951, 16 152. 0872, 4488. 4818, 4979. 4223) was established. The sensitivity and specificity of this model were 100% (41/41) and 100% (20/20) respectively. Conclusion The model with 7 proteins markers has a higher sensitivity and specificity for Kazakh's EC patients in Xinjiang. It may provide a new serum diagnostic tool for Kazakh's EC patients in Xinjiang.
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