鸡胚模型筛选抗血管生成药物的实验方法改进

摘要

目的对传统的鸡胚绒毛尿囊膜(CAM)和卵黄囊膜(YSM)测定法改进，利于评价和筛选抗血管生成的药物。方法在CAM模型中，改变传统的开窗位置、加药载体和结果观察方法；针对YSM模型，改进加药载体和加药部位，优化对实验数据的统计分析；使用重组人血管内皮抑制素endostar和生理盐水对照组评价效果。结果 endostar能明显抑制CAM血管形成，并且抑制YSM的血管密度和面积增长率，与对照组相比有统计学差异(P <0.01)。结论通过本实验对CAM和YSM测定的改进，建立了一个成熟而稳定的筛选抗血管形成药物的实验方法。%Objective To establish improved and universally applicable methods of chick embryo model for anti-angiogenesis drugs screening. Methods We changed traditional window place of chick embryonic chorioallantoic membrane(CAM) assays, model. Drug carrier and methods for observation and statistics analysis were also improved in both arrays. Endostar were used to test and evaluate whether these changes were effective. Results Angiogenesis in CAM and the yolk sac membrane(YSM) assays were significantly inhibited by Endostar, which was consistent with previous reports. The growth rates of blood vessel area and density between Endostar and control groups were also tested and has statistical significance(P <0.01). Conclusion Our results demonstrate that we have established two effective models.These are therefore two very useful and cost effective in vivo models for screening of potential novel therapeutics.