冬虫夏草不同生长阶段甾醇类含量分析

摘要

Sterol is one of the important active substances in Chinese cordyceps, Ophiocordyceps sinensis. In this study, HPLC-ELSD was applied to detect three main sterols, ergosterol, cholesterol and sitosterol, in five different development stages of Chinese cordyceps, namely mycelium, ossified larva, 1cm stroma stage, 4–6cm stroma stage and sporulation stage. Uninfected Hepialus larva sample was set up as experimental control. The results showed that content of total sterols in 4–6cm stroma stage was the highest. This result provides reference for harvesting Chinese cordyceps timely.%冬虫夏草是一味传统名贵中药材,甾醇类成分是其重要的活性成分之一.为了探讨冬虫夏草不同生长阶段甾醇类成分的变化,本研究采用高效液相-蒸发光散射检测器技术对冬虫夏草5个不同阶段样品(冬虫夏草发酵菌丝体、僵虫、1cm子座冬虫夏草,4–6cm子座冬虫夏草,孢子弹射冬虫夏草)和对照样品(蝙蝠蛾幼虫)中麦角甾醇、胆甾醇和谷甾醇的含量进行了分析.结果显示4–6cm子座冬虫夏草样品总甾醇含量较高,该结果为冬虫夏草采收期的选择提供了参考.