鲍姆木层孔菌(桑黄)脂溶性提取物对PC12神经元细胞衰老的保护作用

摘要

To observe the effects of lipophilic extracts from Phellinus baumii on PC 12 aging model induced by NaN3, the cell model was established by NaN3, and then lipophilic extracts from Phellinus baumii were supplied for cell protection. In this model, cell activity, early and late apoptotic rate, and P-galactosidase activity were assayed by MTT, flow cytometry, nuclear staining and the kit for detecting P-galactosidase, aiming to find anti-aging active fraction and effective concentration. The MTT result indicated butanol extracts at the concentration of 200mg/L had better protective effect on activity decrease of PC 12 cell induced by NaN3. In the flow cytometry and nuclear staining experiments, the 200mg/L butanol extracts could inhibit increase of early and late apoptosis rate induced by NaN3. The results also showed the increase in activity of P-galactosidase in PC 12 model could be reduced by 200mg/L butanol extracts. The results indicate extracts of Phellinus baumii can delay the senescence of PC 12 induced by NaN3.%鲍姆木层孔菌(桑黄)Phellinus baumii脂溶性提取物对叠氮钠诱导PC12神经元细胞衰老的保护作用.用叠氮钠作用于体外培养的PC12细胞,并用该菌脂溶性提取物对其进行保护,分别用MTT法、流式细胞仪法、细胞核染色法和β-半乳糖昔酶检测试剂盒检测细胞活力、早期凋亡率、晚期凋亡率和β-半乳糖苷酶阳性细胞率变化,筛选出具有延缓细胞衰老的有效部位和有效作用浓度.通过细胞活力检测发现正丁醇萃取的活性部位可以有效保护叠氮钠诱导的细胞活力下降,其有效工作浓度为200mg/L.流式细胞仪和细胞核染色测定结果均表明200mg/L正丁醇萃取相可以有效抑制叠氮钠引起的PC12细胞早晚期凋亡.B-半乳糖苷酶活性实验结果提示在细胞中添加200mg/L正丁醇萃取物可以有效延缓叠氮钠诱导的神经元细胞衰老.研究结果表明鲍姆木层孔菌正丁醇萃取物可以有效延缓叠氮钠引起PC12细胞衰老.