斑点嗜蓝孢孔菌化学成分、生物活性及水提物荧光猝灭研究

摘要

Different polar fractions were extracted by different reagents from the EtOH-extract of Fomitiporia punctata. Six compounds were isolated and identified as p-sitosterol (1), egosterol (2), erogosta 7,22-dien-3p-ol (3), 5,8-epidioxiergosta-6,22-dien-3p-ol (4), 3-acetoxyoleanolic acid (5) and botulin (6) by the technology of NMR. The anti-tumor activity against NCI-460was evaluated by Alamar Blue method, and the antioxidant activity was detected by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The interactions between aqueous extract and human fibrinogen were investigated by UV/vis absorption and fluorescence spectrum methods. The results showed that the process of binding aqueous extract of F. punctata against human fibrinogen was a spontaneous molecular interaction procedure.%对斑点嗜蓝孢孔菌乙醇提取物进行分级萃取,利用柱层析技术得到6个单体化合物.经核磁数据比对为β-谷甾醇(1)、麦角甾醇(2)、麦角甾-7,22-二烯-3β-醇(3)、5,8-过氧麦角甾-6,22-二烯-3β-醇(4)、3-乙酰齐墩果酸(5)、白桦脂醇(6).采用 Alamar Blue 法检测各有机相及单体化合物对NCI-H460人非小细胞肺癌细胞增殖的影响,用DPPH自由基清除法检测其抗氧化活性.利用荧光光谱及紫外-可见光光谱研究方法在模拟人体生理条件下,研究了斑点嗜蓝孢孔菌水提物与人纤维蛋白原的相互作用,实验结果表明,斑点嗜蓝孢孔菌水提物与人纤维蛋白原的相互形成基态复合物导致人纤维蛋白原内源荧光猝灭.