丹花颗粒对实验性急性心肌缺血大鼠炎症机制的研究

摘要

目的：通过丹花颗粒对急性心肌缺血大鼠炎症因子表达的影响，探讨其保护心肌缺血的作用机制，为进一步研究奠定基础。方法：120只Wistar大鼠，按照随机数字表法分为假手术组、模型组、丹花颗粒高、中、低剂量组、通心络组。灌胃14 d后，以结扎冠脉左前降支建立急性心肌缺血模型。全自动生化仪检测谷草转氨酶（AST）、乳酸脱氢酶（LDH）、肌酸酶同工酶（CK-MB）；记录造模前后ST段变化；双抗夹心酶联免疫吸附方法（ELISA）检测白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、血栓素（TXA2）、前列环素（PGI2）、血清高敏c反应蛋白（CRP）、肌钙蛋白I（Tn-I）浓度；免疫组化方法检测心肌细胞NF-资B表达。结果：造模前后治疗组与模型组比较移ST变化显著（P<0.01）。与模型组比较，丹花颗粒高剂量组与通心络组AST、LDH、CK-MB、Tn-I浓度均明显降低（P<0.05），低剂量组与中剂量组CK-MB、Tn-I浓度明显降低（P<0.05），丹花颗粒组与通心络组CRP表达明显减少（P<0.05），丹花颗粒组与通心络组IL-6、TNF-α浓度明显降低（P<0.05），丹花颗粒组与通心络组PGI2水平均明显增加（P<0.05）；TXA2水平明显降低（P<0.05）。丹花颗粒组及通心络组NF-资Bp65阳性表达明显减弱（P<0.05）。结论：丹花颗粒可能通过抑制NF-资B细胞外的正反馈激活途径，从而减少IL-6、TNF-α、TXA2、PGI2、CRP等炎症因子的基因转录，阻断后者所介导的一系列炎性反应，可能是其防治心肌缺血的作用机制之一。%This study was aimed to explore mechanism of Danhua particles in the protection of myocardial ischemia , and lay the foundation for further study through the expression of inflammatory cytokines of Danhua particles in rats with acute myocardial ischemia . A total of 120 Wistar rats were divided into the sham group , model group, Danhua particles high-dose group, middle-dose group, low-dose group, Tongxinluo group, according to the random number table . The automatic biochemical analyzer was used in the detection of the concentration of the AST , LDH , CK-MB . And the ECG was used to record the ST-segment changes before and after modeling . The ELISA kit was used in the detection of concentration of IL-6 , TNF-α, TXA2 , PGI2 , CRP and TN-I . Immunohistochemical methods were used to detect myocardial cell NF-кB expression . The results showed that before and after the modeling , the treatment group compared with model group ∑ST changed significantly ( P < 0 . 01 ) . Compared with the model group , the Danhua particles high -dose group and Tongxinluo group were significantly reduced in the concentration of AST , LDH , CK-MB and Tn-I ( P <0 . 05 ); the low-dose group and middle-dose group were significantly reduced in the concentration of CK-MB and Tn-I ( P < 0 . 05 ); the expression of CRP in the Danhua particles group and Tongxinluo group was significantly reduced ( P < 0 . 05 ) . Danhua particles group and Tongxinluo group compared with the model group were significantly reduced in the concentration of IL-6 and TNF-α ( P < 0 . 05 ); the PGI2 level in the Danhua particles group and Tongxinluo group was significantly increased ( P < 0 . 05 ); and the TXA2 level was obviously n reduced ( P < 0 . 05 ) . The positive expression of NF-кBp65 in the Danhua particles group and Tongxinluo group was significantly reduced ( P < 0 . 05 ) . It was concluded that Danhua particles inhibited the NF-кB extra cellular positive feedback activation pathway , and thus reduced the inflammatory factor gene transcription such as IL-6 , TNF-α, TXA2 , PGI2 , CRP , block a series of inflammatory reactions which were mediated . It may be one of the mechanisms of Danhua particles in the prevention on myocardial ischemia .