能量代谢仪系统在针灸干预急性酒精性肝损伤中的应用

摘要

目的:探讨能量代谢监测仪系统在针灸改善酒精性肝损伤大鼠能量代谢研究的应用价值.方法:将34只大鼠随机分为正常组、模型组和电针组.模型组和电针组大鼠用50％乙醇给予灌胃,造成急性酒精肝损伤的动物模型.电针组造模后每天给予足三里穴电针30 min治疗一次,共治疗3天.使用动物能量代谢系统对各组动物同期进行连续96 h的观察,全程记录呼吸商(Respiratory ExchangeRatio,RER)、热量、饮食量和饮水量.结果:急性酒精性肝损伤模型大鼠的RER、饮水饮食量显著降低,热量增高;电针干预具有增加RER、饮水饮食量和降低热量值的趋势.结论:将能量代谢监测仪系统应用于针灸干预急性酒精性肝损害大鼠能量代谢的研究表明该仪器系统具有一定的实用价值.%This study aimed to explore the value of animal energy metabolism monitoring system (AEMMS) to evaluate the energy metabolism of acute alcohol liver injury in rat with the treatment of acupuncture.Tirty rats were randomly divided into the control group,the model group and the EA group.The model group and the EA group were gavaged with 50％ ethanol for the establishment of the animal model of acute alcoholic liver injury.EA was given by needling Zusanli (ST-36) 30 min after intragastic administration every day in the EA group.The treatment of acupuncture continued 3 days.In addition,the RER,heat,feeding and drinking were continuously recorded for 96 h in each group at the same time by AEMMS.As a result,the RER,feeding and drinking indices significantly decreased in rats with ALI with an increase in the heat index.There was an up-going tendency of the RER,feeding and drinking indices,and a declined tendency of the heat index in the model rats after EA treatment.It was concluded that AEMMS presented a practical value in the study of the energy metabolism of rats with ALI with the treatment of acupuncture.