鸡新城疫、传染性支气管炎二联活疫苗耐热保护剂筛选和优化

摘要

In this paper, we tested protective effects of 7 groups of the lyoprotectant recipe for Newcastle Disease and Infectious Bronchitis Vaccine, Live Vaccine (La Sota strain + H120 strain) were used for cross reference by an accelerated stability test. The best lyoprotectant rec-ipe of Newcastle-Bronchitis Vaccine had been screened in this experiment, and the results indicat-ed that when exposure this lyoprotectant vaccine at 37 ℃ for 10 days, or exposure at 25 ℃ for 30 days, the vaccine virus titers declined by 0.4 unit or 0.4~0.6 unit respectively, while other lyo-protectant vaccines virus titers decreased by 2~2.4 unit or 1.8~2.6 unit respectively. In conclu-sion, the new lyoprotectant showed effective protection for Newcastle-Bronchitis Vaccine.%应用冻干制品加速热稳定试验，测试7组冻干保护剂配方对鸡新城疫、传染性支气管炎二联活疫苗（La Sota株+H120株）的耐热保护效果。试验筛选出用于鸡新城疫、传染性支气管炎二联活疫苗的耐热保护剂，使用该保护剂的疫苗表现为37℃保存10 d，病毒含量下降0.4个滴度，常规疫苗下降2～2.4个滴度；25℃保存30 d，病毒含量下降0.4～0.6个滴度，常规疫苗下降1.8～2.6个滴度。证明此保护剂具有良好的耐热效果。