A LC-MS/MS method to fast determine tanshinone 1IA in human plasma was established. The internal standard (cryptotanshinone) 20μL was added to 20GuL plasma,then precipitated with 580nL acetonitrile. After cenlrifugelling and transfering and evaporating,the residue was dissolved with 400uL 10% acetonitrile and determined by injecting 5μL to LC-MS/MS system. ASB C18 column was used and the mobile phase consisted of acetonitrile- water gradient system at the flow rate of 0.5mL/min. MS determination was in electrospray ionization (ESI) positive mode and in multi-reaction monitoring (MRM) mode. The ion pair in quantitative analysis was m/z 295.1 →mlz 249.2(tanshinone IIA), m/z 297.2→m/z 221.1 (cryptotanshinone, internal standard), respectively. The linear range was 0.2~800ng/mL (r =0.9974), and the limit of detection for tanshinone Hawas 0.2 ng/mL. The method is simple, rapid, sensitive, and reliable, and it is suitable for the determination of drug concentration of tanshinone IIA in human plasma.lt also can provide some reference for clinical pharmacokinetics research.%建立测定人血浆中丹参酮HA含量的LC-MS/MS方法.取正常人血浆200μL,然后加入200ng/mL隐丹参酮溶液(内标)20μL,加入580μ L乙腈沉淀,离心10min( 15000rpm)后,取上清液吹干溶剂后,用10％乙腈400μL溶解,取5μL进行LC-MS/MS测定.LC条件:采用ASB C18柱(2.1×50mm,5μm),流动相:乙腈-水梯度洗脱,流速:0.5mL/min.质谱条件:ESI电离源,正离子模式,多反应监测(MRM)方式,用于定量分析的离子对分别为m/z 295.1 →m/z 249.2(丹参酮IIA)和m/z 297.2→221.1(内标,隐丹参酮).该方法丹参酮IIA的线性范围为0.4～800ng/mL,最低检测限为0.2ng/mL.本方法操作简便、快速、结果准确、可用于该药物的含量测定,同时也可为临床药代动力学研究提供参考.
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