畲药搁公扭根的质量标准研究

摘要

Objective:To improve the quality standard of traditional she medicine—Ge gongniugen.Methods:The microscopical characteristics were identified by microscopical method,and index ingredient (Gallic acid)were determined by HPLC.The water content,total ash and ethanol-soluble extractives were determined according to the method in appendix of"pharmacopoeia of people’s republic of china".Results:Characteristics of microscopical identification of Ge gongniugen were obviously.A good linearity of psoralen was at 3.881 ~38.81 μg·mL -1 (r =0.999 7),and the average recovery rate was 99.47% (n =6),RSD =1.22%.The content of gallic acid in 14 batches of Ge gongniugen was in the range of 0.28%-1.46%.The results of water content,total ash and ethanol-soluble extractives were in the range of 5.0%-10.2%,1.4%-3.6% and 14.4%-41.8%,respectively.Conclusion:This method is simple,accurate,applicable for the quality control of Ge gongniugen.%目的：完善畲药“搁公扭根”的质量标准。方法：运用显微鉴别法、高效液相色谱法分别对搁公扭根的粉末显微特征、指标性成分（没食子酸）进行测定；参照《中华人民共和国药典》2010版附录相关方法对搁公扭根水分、总灰分和醇溶性浸出物进行测定。结果：搁公扭根粉末显微特征明显；没食子酸的线性范围为3.881～38.81μg·mL －1（r ＝0.9997），平均加样回收率为99.47％（n ＝6），RSD ＝1.22％。14批搁公扭根样品的测定结果表明，没食子酸的含量为0.28％～1.46％，水分、总灰分和醇溶性浸出物分别为5.0％～10.2％、1.4％～3.6％和14.4％～41.8％。结论：研究所得方法简便、准确，可用于搁公扭根的质量控制。