药用植物短蕊万寿竹的组织培养研究

摘要

Objective:The aim of the research was to develop and utilize Disporum brachystemon Wang et Tang better.Methods:To investigate the effects of different hormone combinations on cluster bud induction and root culture of Disporum brachystemon Wang et Tang.By orthogonal design,stem was used as the explants and the MS was used as the basic culture medium.Results:The results indicated that the best initial medium was MS + 6-BA 1.0 mg· L-1 + NAA 0.2 mg· L-1 + IBA 0.5 mg·L-1.The most effective medium for cluster bud induction was MS + 6-BA 1.5 mg· L-1 + NAA 0.2 mg· L-1 + KT 1.0 mg· L-1,and the multiplication coefficient of cluster bud reached 6.88.The best rooting medium was MS + 6-BA 0.5 mg·L-1 + NAA 2.0 mg· L-1 + AC 2.0 g·L-1,and roofing ratio was above 96％.Conclusion:The tissue culture and propagation system was astablished and solve the shortage of large-scale planting.%目的:更好的开发利用短蕊万寿竹.方法:以短蕊万寿竹的幼嫩茎段为外植体,MS为基本培养基,采用正交设计研究外源激素的种类和配比对诱导丛生芽和生根的影响.结果:最适的启动培养基为MS +6-BA 1.0 mg·L-1+NAA0.2 mg·L-1+IBA0.5 mg·L-1,诱导丛生芽的最佳培养基为MS +6-BA 1.5 mg·L-1+NAA 0.2 mg·L-1+KT 1.0 mg·L-1,丛生芽的增殖系数达到6.88,最佳的生根培养基为MS+ 6-BA 0.5 mg· L-1+ NAA2.0 mg·L-1+AC 2.0 g·L-1,生根率达到96％以上.结论:建立短蕊万寿竹的组织培养繁殖体系,为人工种植提供大量种苗.