目的:建立牛大力中刺桐碱和高丽槐素含量的测定方法.方法:采用超声法提取药材,通过对提取工艺及色谱条件进行优化建立测定方法,同时测定牛大力中刺桐碱、高丽槐素的含量.HPLC色谱条件为色谱柱C18 (250 mm ×4.6 mm, 5 μm),流动相为乙腈-0.1％冰醋酸水溶液梯度洗脱,检测波长为280 nm和310 nm,流速为 1.0 mL·min1,柱温为30 ℃.结果:刺桐碱进样量在0.284 4 ～ 1.422 0 μg与峰面积值呈现良好的线性关系(r= 0.999 9),平均回收率为99.6％, RSD为1.57％ (n =6);高丽槐素进样量在0.087 0 ～ 0.435 2 μg与峰面积值呈现良好的线性关系(r = 0.999 8),平均回收率为100.9％, RSD=2.18％(n=6).结论:该方法准确、简便、重复性好,可用于牛大力药材中刺桐碱和高丽槐素的含量测定.%Objective: To establish an HPLC method for the determination of hypaphorine and maackiain in root of Millettia speciosa. Methods: Ultrasonic method was used to extract the herbs, through the extraction process and chromatographic conditions, the method for simultaneous determination of maackiain and hypaphorine in root of Millettia speciosa was optimized. HPLC chromatographic conditions included the C18 (250 mm ×4.6 mm, 5 μm) column and the mobile phase was eluted with a gradient of acetonitrile-0.1％ acetic acid solution. The flow rate was 1.0 mL · min-1 and the detection wavelength was set at 280 nm and 310 nm. The column temperature was maintained at 30 ℃. Results: The calibration curve of hypaphorine was in good linearity over the range of 0.284 4-1.422 0 μg (r = 0.999 9). The average recovery was 99.6％ with RSD of 1.57％ (n =6). And the calibration curve of maackiain was in good linearity over the range of 0.087 0-0.435 2 μg (r = 0.999 8). The average recovery was 100.9％ with RSD of 2.18％ (n = 6). Conclusion: The method is accurate, simple, reproducible, and can be used to determine the content of hypaphorine and maackiain in the root of M. speciosa.
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