地瓜参总皂苷含量测定方法研究

摘要

以人参皂苷Re为标准对照,香草醛—冰醋酸—高氯酸作为显色体系,建立地瓜参皂苷含量测定方法,结果表明：皂苷浓度在40～200μg/mL时,与吸光度值呈良好线性关系,加标回收率为101.9%,RSD=4.63%,精密度试验RSD=1.77%,稳定性试验RSD=3.16%,重复性试验RSD=3.15%,说明方法学各指标均符合要求。%A quantitative analysis of the Acaudina molpadioides method was established by using the ginseng saponins Re as standard and vanillin—glacial acetic acid—high chlorine acid as color-forming reagents.The results showed that concentration had a good relationship with the absorbance when it was among 40-200 μg/mL.The average recovery was 101.9%,RSD=4.63%.Precision experiments RSD=1.77%,stability experiments RSD=3.16%,repetitive experiments RSD=3.15%,explained that the indexes of methodology met the requirements.