不同浓度甘油冷冻保护剂和冷冻麦管规格对七彩鲑受精率的影响

摘要

以5％-30％浓度的甘油作为精液冷冻保护剂，以0．5mL麦管和1．8mL麦管作为精液冻存容器，进行七彩鲑冻精的受精试验。试验结果表明：20％浓度甘油组的受精率达到了51．08％～61．56％，显著高于其他浓度甘油组的受精率；在20％～25％甘油浓度中0．5mL麦管组的受精率均高于1．8mL麦管组的受精率，其中20％甘油的0．5mL麦管组，平均受精率最高，达到了61．38％。%The artificial fertilization experiment on Salvelinusfontinalis was carried out,using 5%-30% glycerol as eryoproteetant,and 0.5 mL and 1.8 mL straw as semen eryopreservation container. The results showed that：The fertilization rates of 20% glycerol group reached 51.08%-61.56%, which was obviously higher than other concentrations of glycerol groups. With the glycerol concentration of 20%-25% ,the fertilization rates of 0.5 mL straw group were significantly higher than the fertilization rates of 1.8 mL straw group. The mean fertilization rate of 20% glycerol and 0.5 mL straw group was the highest ,which reached 61.38%.