Spred2基因对人肝癌细胞凋亡的影响

摘要

目的 观察Spred2对人肝癌细胞(HepG2)凋亡的影响.方法 用阳离子脂质体瞬时转染 pcDNA3.0,pcDNA3.0-Spred2到HepG2细胞,建立过表达Spred2的细胞模型.膜联蛋白(annexin)Ⅴ-FITC /PI(7-AAD)双标通过流式细胞仪检测细胞凋亡;蛋白质印迹检测Spred2的表达水平.结果 转染Spred2基因能明显诱导HepG2细胞凋亡.另外,转染Spred2基因能显著地增强5-FU诱导HepG2细胞凋亡.结论 Spred2对人肝癌细胞凋亡有调控作用.%Objective To observe the effect of Spred2 on apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were transfected with pcDNA3. 0 and pcDNA3. O-Spred2 for the morphologic change of HepG2 to be observed. Cells were stained with annexin V conjugated to fluorescein isothiocyanate ( annexin V -FITC ) and propidium iodide ( PI/ 7-AAD ). The stained cells were analyzed on a flow cytometer using the Cellquest program. The expression level of Spred2 was examined by Western blot. Results Spred2 over-expression induced HepG2 cells apoptosis and sensitized cells to 5-FU induced HepG2 cells apoptosis. Conclusions Spred2 can regulate HepG2 cellular apoptosis.