目的 利用原子力显微镜(AFM)观察支撑磷脂膜的微观形成过程.方法 冰浴超声法制备磷脂脂质体溶液,囊泡融合法形成人工磷脂双分子层,用AFM进行观察.结果 利用AFM观察到磷脂膜的微观形成过程为:脂质体囊泡吸附→脂质体囊泡之间发生黏着→脂双层之间形成接触点→磷脂囊泡破裂→形成磷脂分子层→磷脂分子层间流动进行融合形成大片磷脂膜.结论 AFM可观察磷脂膜的微观形成过程,进而为细胞膜和膜蛋白的功能研究提供基础.%Objective To observe the microscopic forming procedure of phospholipid membrane by AFM. Methods Ice-bath ultrasound was used to prepare liposome solution. Ves-fusion technique was applied to form the phospholipid membrane on mica. AFM was used to observe the forming procedure of phospholipid membrane. Results The microscopic forming procedure of phospholipid membrane was observed by AFM, which was from the adsorption of liposomes,adherence,the formation of the point of touching between the phospholipid layers to the ruptures of liposomes, the formation of big phospholipid layers and to the fusion among these layers. Conclusion AFM can be used to observe the microscopic forming procedure of phospholipid membrane, which contributes to further studies on the function of membrane and membrane protein.
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