Assemblies of polymerized phospholipid vesicle structures chemisorbed on Au films visualized in situ by environmental scanning electron microscopy

摘要

Submicron vesicles immobilized on gold films were visualized in situ using environmental scanning electron microscopy (ESEM). Electron micrographs show that surface immobilized vesicles composed of diacetylenic phospholipids with 1 mole percent disulfide functionality and that encapsulate NaCl are structurally stable for at least three days. Furthermore, energy dispersive spectroscopy (EDS) provides compositional evidence supporting the formation of surface immobilized vesicles. Using ESEM coupled with EDS, a two-layer vesicle structure was imaged and found to contain NaCl and lipid elements sulfur and phosphorous.