肺炎克雷伯菌TH6菌株的生物学特性及生物膜形成分析

摘要

目的 从临床炎症性肠炎患者粪便中分离1株肺炎克雷伯菌株(Klebsiella pneumoniae),探究其生物学特性及生物膜形成的特点.方法 对TH6菌株进行16S RNA鉴定、MALDI-TOF的质谱鉴定以及全自动生化检测,确定为肺炎克雷伯杆菌.对其形态,生长速率,生物膜形成,荚膜毒力因子等生物学特性进行分析.结果与结论 在透射电镜下TH6菌株为短杆状,具有荚膜,荚膜血清型实验结果显示为K57型. K.pneumonia TH6在有氧条件下生长速度非常快,为标准菌株ATCC2146和ATCC1705的1.6倍,在1 h时即可达到对数生长期. 在激光共聚焦显微镜下分别观察4、8、12、24、48、72、96和120 h内的生物膜形成状况,发现该菌株在培养72 h后形成生物膜.K.pneumonia TH6的生长速度快、生物膜的形成等特点均表明该菌适应新环境能力强,一旦在体内定植则难以根治.%Objective To explore the biological characteristics and biofilm formation of a strain of Klebsiella pneumoniae isolated from the stool of a patient with clinical inflammatory bowel disease .Methods The TH6 strain was identified as Klebsiella pneumoniae by 16S RNA, MALDI-TOF and automatic biochemical instrument .The morphology, growth rate, biofilm formation, capsid virulence factors and other biological characteristics were analyzed .Results and Conclusion The TH6 strain was short rod-shaped with a capsule under the transmission electron microscope , and was of K57 type according to the capsule serotype test .K.pneumonia TH6 grew rapidly under aerobic and anaerobic conditions , almost at twice the rate of standard strain ATCC2146, and reached the logarithmic growth phase at 2 h.The strain had multi-drug resistance.Biofilm formation was observed under a confocal laser scanning microscope (CLSM) at 4, 8, 12, 24, 48, 72, 96 and 120 h and the biofilm was formed at 72 h.The ability of fast growth , biofilm formation and other characteristics of K.pneumonia TH6 showed that this strain has a strong ability to adapt to the new environment is difficult to cure after colonization in vivo.