Objective To study the effects of oleuropein on irradiation-induced ovarian injury. Methods 32 femal SD rats were randomly assigned to 4 groups of 8 rats each: control group, oleuropein group, irradiation group and irradiation + oleuropein group. Rats in control group and irradiation group received distilled water 10 mL/( kg·d) intragastrically. Rats in oleuropein group and irradiation + oleuropein group received oleuropein 100 mg/( kg·d) intragastrically. 12 weeks later, rats in group irradiation group and irradiation + oleuropein group were exposed to pelvic irradiation at a dose of 5 Gy when they were in the estrus. After 7 days for radia-tion, the concentration of AMH, IL-1, IL-6 and TNF-αwere detected and the number of growing follicles in ovarian tissue were coun-ted. Results Compared with the control group, the serum AMH concentration in the radiation group was significantly decreased ( P<0. 05) , and IL-1, IL-6 and TNF-α were increased ( P<0. 05) and the number of follicles in the ovaries was reduced. Compared with the radiation group, the serum AMH concentration were increased in the radiation + peridol group, and IL-1, IL-6 and TNF-α de-creased ( P<0. 05) . The total number of follicle was increased. Conclusion It was concluded that oleuropein could inhibit the radia-tion-induced inflammatory response to reduce the radiation damage of the ovaries.%目的 探讨橄榄苦甙减轻卵巢辐射损伤的效果及其机制.方法 32只成熟雌性SD大鼠随机分为4组,正常对照组和辐射组予蒸馏水10 mL/(kg·d),橄榄苦甙组和辐射+橄榄苦甙组予橄榄苦甙100 mg/(kg·d),连续灌喂12周.12周后,辐射组和辐射+橄榄苦甙组大鼠动情期时,接受5 Gy的X线盆腔照射1次.辐射后7 d检测血清抗苗勒氏管激素(AMH)、IL-1、IL-6和TNF-α含量,计算卵巢组织各级卵泡数.结果 与正常对照组比较,辐射组血清AMH浓度显著降低(P<0.05),血清IL-1、IL-6、TNF-α浓度升高(P<0.05);卵巢中卵泡数减少.与辐射组比较,辐射+橄榄苦甙组血清AMH升高,IL-1、IL-6、TNF-α浓度降低(P<0.05);卵泡总数增加.结论 橄榄苦甙可通过抑制辐射诱导的炎症反应,减轻卵巢的辐射性损伤.
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