不同微生物的α-乙酰乳酸脱羧酶的生理生化特性的研究

摘要

分析测定了不同微生物的α-乙酰乳酸脱羧酶(ALDC)酶活力，结果表明不同来源的ALDC酶活力有较大差异，酶反应速度曲线存在明显差异；酶反应体系的pH对ALDC酶活力有明显的影响，如乳酸乳球菌的ALDC酶反应最适pH为6.6，而产气气杆菌的ALDC酶反应的最适pH为5.8；酶反应体系中加入不同浓度的亮氨酸、缬氨酸和异亮氨酸对不同来源的ALDC酶活性有较明显的影响。%The enzyme activity of α－Acetolactate Decaroboxylases (ALDC)from different microbes was studied, the results demonstrated that it was quite different among them. There were diversities of their enzyme reaction velocities. It was clear that the enzyme activity was affected by the pH of the enzyme reaction system, for example, the optimum pH of ALDC from Lactococcus lactis was 6.6, while for Aerobacter Aerogenes it was 5.8. Addition leucine,valine and isoleucine into enzyme reaction system obviously affected the enzyme activity of ALDC from different microbes.